Mass Spec and Proteomics > Rates & Forms


Please choose the correct electronic form below based upon your affiliation to OSU:
On-Campus
Off-Campus Consortium (Non-OSU Ohio Universities)
Off-Campus Non-Ohio University and Government
Off-Campus Industry

Please Note - New Electronic Service Forms

Please only submit service requests when you are ready to submit/send samples.

For Internal (OSU) Users
In order to use FOM you need to have an OSU name.# account and must fill out a profile as well as add billing account information before you can submit a sample for the first time. Refer to this PDF for a step by step process for creating a FOM account and using the system.

For External Users:
          Directions to submit a Service Request Form and samples to MS&P Faclity

          OSU Sponsored Guest Account / FOM Profile and Account Form

FACILITY ONLINE MANAGER (FOM) http://fom.osu.edu
Go to FOM to: add/inactivate existing account information, change profile information, view your Forms submitted to MS&P Facility, view billing information.

Sample Submission:
Please use the drop down above and you will be directed to the proper service request submission link.

When submitting multiple samples, please fill out only one (1) Service Request Form in FOM and attach all sample information in a separate document. Please do not submit multiple forms. If you run into problems with FOM please contact fom-admin@osu.edu.


Mass Spectrometry & Proteomics Facility -
FY17 BILLING RATES (Effective October 1st, 2016)

Unit
OSU/
Consortium
Non-Ohio
University & Government
Industry
Molecular weigth/Chemical Formula determination
Accurate mass (MaXis and FT-ICR)
Sample $35.00 $60.00 $120.00
Nominal mass (Amazon, MaXis, UltrafleXtreme (TOF-TOF) Sample $30.00 $50.00 $80.00
         
Metabolomics        
Method development:        
Qualitative/untargeted (GC-MS) Project $500.00 $900.00 $1500.00
Quantitative/targeted (GC-MS) Project $500.00 $900.00 $1500.00
Qualitative/untargeted (LC-MS) Project $1000.00 $1800.00 $3000.00
Quantitative/targeted (LC-MS) Project $1200.00 $2000.00 $3500.00
         
Sample Analysis:        
GC-MS injection (< 10 samples, each injection) Injection $80.00 $150.00 $300.00
GC-MS injection (>10 samples, each injection) Injection $80.00 $150.00 $300.00
LC-MS injection (< 10 samples, each injection), quantitative Injection $120.00 $180.00 $250.00
LC-MS injection (>10 samples, each injection), quantitative Injection $100.00 $150.00 $220.00
LC-MS injection (< 10 samples, each injection), qualitative Injection $140.00 $280.00 $400.00
LC-MS injection (>10 samples, each injection), qualitative Injection $120.00 $200.00 $300.00
         
Imaging/Tissue        
Large molecules (>10,000 Da) MALDI TOF-TOF Sample $750.00 $1000.00 $2000.00
Small molecules (<10,000 Da), high resolution FT-ICR Sample $750.00 $1000.00 $2000.00
         
Self operation and Staff time (per hour)        
Self operation LTQ and Amazon (min 1/2 hr increments) Hour $5.00 $15.00 $50.00
Self operation GC MS and MALDI TOF (min 1/2 hr increments) Hour $10.00 $30.00 $80.00
Self operation Orbis, ICR (min 1/2 hr increments) Hour $20.00 $50.00 $120.00
Staff time Hour $50.00 $100.00 $200.00
         
Proteomics        
1D SDS-PAGE - 10 Wells Sample $60.00 $100.00 $200.00
2D SDS-PAGE - Large Gel $120.00 $200.00 $300.00
2D SDS-PAGE - Mini Sample $60.00 $100.00 $150.00
Albumin Removal or Peptide/Protein Enrichment Sample $240.00 $350.00 $600.00
Cell Lysis Sample $50.00 $80.00 $150.00
Coommassie Stain (Includes Image Acquisition) Gel $60.00 $120.00 $300.00
Sypro Ruby/Deep Purple/Multiplex Stain (Incl Image Acquisition) Gel $240.00 $480.00 $800.00
DIGE/iTRAQ Labeling Sample by quote by quote by quote
Enzyme Digestion <20 Samples $30.00 $50.00 $80.00
Ettan Coring & Digestion - 96 Well Plate Sample $150.00 $200.00 $300.00
Protein Precipitation Sample $20.00 $40.00 $100.00
Protein Quantitation (Bradford) Sample $20.00 $50.00 $150.00
Cryomill Sample $5.00 $20.00 $50.00
Lyophylizer Sample $0.00 $10.00 $20.00
         
Sample Analysis:        
Data Processing (PTM, Seq Anal, iTRAQ, Labl Free Bioinformatics) Project $600.00 $1200.00 $2000.00
LC-MS/MS Peptide Sequencing - 1D/SCX Fractions - XL Sample $450.00 $600.00 $1000.00
LC-MS/MS Peptide Sequencing - 1D/SCX Fractions - Fusion/QE Sample $600.00 $900.00 $1400.00
LC-MS/MS Peptide Sequencing - Long Gradient (> 2hrs) - XL Sample $250.00 $320.00 $400.00
LC-MS/MS Peptide Sequencing - Long Gradient (> 2hrs) - Fusion/QE Sample $300.00 $500.00 $800.00
LC-MS/MS Peptide Sequencing - Reg Gradient (< 2hrs) - XL Sample $120.00 $200.00 $300.00
LC-MS/MS Peptide Sequencing - Reg Gradient (< 2hrs) - Fusion/QE Sample $150.00 $220.00 $350.00
LC-MS/MS Peptide Sequencing - 2D LC Fractions (per fraction) - XL Fraction $60.00 $100.00 $200.00
LC-MS/MS Peptide Sequencing - 2D LC Fractions (per fraction) - Fusion/QE Fraction $75.00 $150.00 $300.00
Top Down Proteomics (Fusion, QE or ICR) Sample $500.00 $800.00 $1500.00
         
Protein Expression & Purification        
Cloning of DNA into Competent Cell Line Clone $400.00 $800.00 $2000.00
Column Chromatography Sample $300.00 $600.00 $1500.00
IP with Antibody Provided Sample $200.00 $400.00 $800.00
Large-Scale Purification (Researcher Provides Clone) Clone $1200.00 $1800.00 $2500.00
Protein Expression & Optimization Clone $300.00 $600.00 $1200.00
Small Trial Purification Clone $300.00 $650.00 $1500.00
Western Blot Gel $200.00 $400.00 $750.00
         

Sample Submission Guidelines

Samples can be brought to 250 Biomedical Research Tower, along with a filled out sample submission form, weekdays between 8 am and 5 pm. We have a refrigerator, freezer and dry box for samples that require special storage. It is preferred if samples are submitted neat or dried down, typically ug amounts is all that is needed.

Biological samples are handled differently and require different conditions. Salts, buffers and surfactants suppress ionization and need to be removed for mass spec analysis. When possible, it is advised to dialyze the sample into nanopure or at least distilled water prior to submission. We also have several de-salting techniques for proteins and oligonucleotides available in our facility. List all salts, buffers and detergents the protein or oligonucleotide is in, or has recently been in. For optimal results, please discuss your project with Dr. Somogyi prior to submission. Certain classes of proteins need to be handled differently than others, the more we know about your system, the better the data. Protein samples for ESI are prepared in 50% acetonitrile 50% water with 0.1% formic acid. Slightly acidic conditions are necessary to facilitate protonation

We CANNOT run samples that are in aromatic solvents like benzene, toluene, or solvents such as DMSO and DMF.

Electrospray and MALDI samples are prepared in Methanol, Acetonitrile, water, THF, chloroform. We will acidify the sample with acetic or formic acid unless you indicate the sample is acid sensitive.

Turn around times vary between 1 day to a week depending on sample load, instrument availability and type of analysis. We will email you when the results are ready to be picked up.