For publication experimental write ups, please contact Dr. Kari Green-Church
 

Our protocol has been adapted from the method described by Wilm and Mann and is similar to protocols used by a number of investigators.  The following papers are good references. 

 

Anal. Chem.  68:850-858, 1996,

Biol. Reproduct. 61:1184-1197, 1999

J. Biol. Chem. 274:16501-16507, 1999

Proc. Natl. Acad. Sci. U.S.A. 96:6591-6596, 1999

J. AM. Soc. Mass Spectrom. 9:194-201, 1998

Anal. Biochem.  263:93-101, 1998

 

A Great Reference book is:

Protein Sequencing and Identification Using Tandem Mass Spectrometry  Michael Kinter and Nicholas E. Sherman.  Wiley Interscience 2000.

Here is a general description of our procedure.  

 

 

 

 

 


 

 

 

 

 

 

 

 

Proteomics at The Ohio State University | A Proteomic Experiment | Example of Data | Instrumentation at the CCIC-MS Laboroatory | Staff and Location | Sample Submission Procedures | Coomassie Stain Protocol | In-Gel Digestion Protocol | Fee Structure

To contact us:

Phone: 614-292-4821
Fax: 614-292-5955
Email: kgreenchurch@ccic.ohio-state.edu