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Accurate identification of lipids in biological samples is a key step in lipidomics studies. Multidimensional nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical tool for this purpose as it provides comprehensive structural information on lipid composition at atomic resolution. However, the interpretation of NMR spectra of complex lipid mixtures is currently hampered by limited spectral resolution and the absence of a customized lipid NMR database along with user-friendly...

1. Prof. Jim Prell, University of Oregon “Quantitative Protein Unfolding with a Q-TOF” Date: February 24th Time: 5:30 PM Place: 105 BRT 

A new non-cytotoxic [(+)-17β-hydroxystrebloside (1)] and two known cytotoxic [(+)-3'-de-O-methylkamaloside (2) and (+)-strebloside (3)] cardiac glycosides were isolated and identified from the combined flowers, leaves, and twigs of Streblus asper collected in Vietnam, with the absolute configuration of 1 established from analysis of its ECD and NMR spectroscopic data and confirmed by computational ECD calculations. A new 14,21-epoxycardanolide (3a) was synthesized from 3 that was treated with...

A new non-cytotoxic [(+)-17β-hydroxystrebloside (1)] and two known cytotoxic [(+)-3'-de-O-methylkamaloside (2) and (+)-strebloside (3)] cardiac glycosides were isolated and identified from the combined flowers, leaves, and twigs of Streblus asper collected in Vietnam, with the absolute configuration of 1 established from analysis of its ECD and NMR spectroscopic data and confirmed by computational ECD calculations. A new 14,21-epoxycardanolide (3a) was synthesized from 3 that was treated with...

1. Dr. Ralph Tobias, Hummon Group “Developing a Drug Screening Platform: MALDI-Mass Spectrometry Imaging of Paper-Based Cultures” 2. Ms. Haley Chatelaine, Kopec Group “A Targeted and Untargeted LC-MS Approach to Determining Postprandial Vitamin A&D and Lipid Levels in Metabolic Syndrome versus Healthy Subjects” Date: January 21st Time: 5:30 PM Place: 105 BRT

BACKGROUND: Accurate whole-body staging following biochemical relapse in prostate cancer is vital in determining the optimum disease management. Current imaging guidelines recommend various imaging platforms such as computed tomography (CT), Technetium 99 m (^(99m)Tc) bone scan and ^(18)F-choline and recently ^(68)Ga-PSMA positron emission tomography (PET) for the evaluation of the extent of disease. Such approach requires multiple hospital attendances and can be time and resource intensive....

1. Dr. Djawed Bennouna, Kopec Research Group, OSU “The Effects of Omega-3 Supplementation on the Brain Lipids after Chemotherapy” 2. Dr. Dmytro Kulyk, Badu Research Group, OSU “High-Throughput Mass Spectrometry Platform for Gas-Phase Reaction Screening under Ambient Conditions” Date: November 26 Time: 5:30 PM Place: 105 BRT

There will be a special seminar next week by Dr. Debra Rolison from the Naval Research Laboratory. Title: “Science is Too Important to be Left Just to Men” Place: E0040 Date: Oct 22, 2019 Time: 5 – 6 pm Since it coincides with our October meeting, MSDG is canceled for this month. This seminar will […]

NMR spectroscopy is an extraordinarily rich source of quantitative dynamics of proteins in solution using spin relaxation or chemical exchange saturation transfer (CEST) experiments. However, ^(15)N-CEST measurements require prolonged multidimensional, so-called pseudo-3D HSQC experiments where the pseudo dimension is a radio frequency offset Δω of a weak ^(15)N saturation field. Nonuniform sampling (NUS) approaches have the potential to significantly speed up these measurements, but they also...

NMR spectroscopy is an extraordinarily rich source of quantitative dynamics of proteins in solution using spin relaxation or chemical exchange saturation transfer (CEST) experiments. However, ^(15)N-CEST measurements require prolonged multidimensional, so-called pseudo-3D HSQC experiments where the pseudo dimension is a radio frequency offset Δω of a weak ^(15)N saturation field. Nonuniform sampling (NUS) approaches have the potential to significantly speed up these measurements, but they also...

Topic: Thermal ionization mass spectrometry methods and applications in the earth and ocean sciences Overview by Prof. Elizabeth Griffith, School of Earth Sciences, OSU Application by Ms. Samantha Carter, Griffith Research Group, OSU Date: September 24 Time: 5:30 PM Place: JAMES B050 (460 W. 10TH Ave)

Please join us for the 3rd Annual Ohio Mass Spectrometry and Metabolomics Symposium (OMSMS) to be held at the Blackwell Inn and Pfahl Conference Center at The Ohio State University in Columbus, Ohio, on October 1-2, 2019. Invited keynote and plenary presentations: Connecting the World’s Mass Spectrometry Data to Understand the Chemistry of Life — a Big […]

“Panoptic Mass Spectrometry: How and Why” Date: Sept 16, 2019 Time: 4:10 PM Place: 130 CBEC

Protein function depends critically on intrinsic internal dynamics, which is manifested in distinct ways, such as loop motions that regulate protein recognition and catalysis. Under physiological conditions, dynamic processes occur on a wide range of time scales from subpicoseconds to seconds. Commonly used NMR spin relaxation in solution provides valuable information on very fast and slow motions but is insensitive to the intermediate nanosecond to microsecond range that exceeds the protein...

Protein function depends critically on intrinsic internal dynamics, which is manifested in distinct ways, such as loop motions that regulate protein recognition and catalysis. Under physiological conditions, dynamic processes occur on a wide range of time scales from subpicoseconds to seconds. Commonly used NMR spin relaxation in solution provides valuable information on very fast and slow motions but is insensitive to the intermediate nanosecond to microsecond range that exceeds the protein...

Mr. Taghi Sahraeian, Badu Research Group, OSU “Droplet Imbibition Enables Non-Equilibrium Interfacial Reactions in Charged Microdroplets” Date: August 27th Time: 5:30 PM Place: 105 BRT COH-MSDG – Central Ohio (OH) Mass Spectrometry Discussion Group

Mowei Zhou will present a seminar while visiting us from Pacific Northwest National Laboratory next week: “Deciphering Proteins with Unknown Functions in Plants using Top-Down and Native Mass Spectrometry Techniques” Tuesday, August 20, 2019 4:00 pm BRT 105 Mass spectrometry (MS) has been widely used in proteomics applications for identifying and quantifying proteins in biological […]

Oral cancer is a public health problem with an incidence of almost 50,000 and a mortality of 10,000 each year in the USA alone. Black raspberries (BRBs) have been shown to inhibit oral carcinogenesis in several preclinical models, but our understanding of how BRB phytochemicals affect the metabolic pathways during oral carcinogenesis remains incomplete. We used a well-established rat oral cancer model to determine potential metabolic pathways impacted by BRBs during oral carcinogenesis. F344...

Oral cancer is a public health problem with an incidence of almost 50,000 and a mortality of 10,000 each year in the USA alone. Black raspberries (BRBs) have been shown to inhibit oral carcinogenesis in several preclinical models, but our understanding of how BRB phytochemicals affect the metabolic pathways during oral carcinogenesis remains incomplete. We used a well-established rat oral cancer model to determine potential metabolic pathways impacted by BRBs during oral carcinogenesis. F344...

A special seminar will be presented by Dr. Michael T. Marty (University of Arizona) on Uncovering Antimicrobial Peptide Interactions in Lipid Nanodiscs by Native MS. Tuesday, June 25th 10:00-11:00 am BRT 105 Research in the Marty lab is focused on developing mass spectrometry methods to study the structure and biophysics of membrane proteins. Working at […]