Please choose the correct ELECTRONIC SERVICE REQUEST FORM below based upon your affiliation to OSU:
On-Campus
Off-Campus Consortium (Non-OSU Ohio Universities) **
Academic/Non-profit (Outside OH Consortium)
Off-Campus Industry
** NCH users should use "Off-Campus Consortium (Non-OSU Ohio Universities)" as they are eligible for internal/consortium rate structure
FY2024 BILLING RATES (Effective October 1, 2023)
|
|
Unit | OSU/ Consortium * includes NCH |
Academic/Non-profit (Outside OH Consortium) | Industry |
|---|---|---|---|---|
| Molecular weight/Chemical Formula determination | ||||
| Accurate mass (FT-ICR and XL) | Sample | $55.00 | $110.00 | $190.00 |
| Nominal mass (Amazon, UltrafleXtreme (TOF-TOF) | Sample | $40.00 | $75.00 | $100.00 |
| Metabolomics | ||||
| Method development | ||||
| Quantitative/Targeted (LC-MS) | Project | $1,200.00 | $1,700.00 | $2,100.00 |
| Qualitative/Untargeted (LC-MS) | Project | $1,300.00 | $1,900.00 | $2,600.00 |
| Metabolite extraction/sample clean up | Hours | $55.00 | $110.00 | $120.00 |
| Sample Analysis | ||||
| LC-MS/MS Targeted (Quantiva) | Injection | $120.00 | $160.00 | $300.00 |
| LC-MS/MS Untargeted (QE-M, Agilent) | Injection | $140.00 | $220.00 | $350.00 |
| Imaging/Tissue | ||||
| Imaging, by staff (MALDI TOF-TOF, FTICR) | Hours | $55.00 | $110.00 | $220.00 |
| Imaging, small molecules by staff (<10,000 Da), high resolution (timsTOF flex) | Hours | $55.00 | $110.00 | $220.00 |
| Use of Ambergen probes for imaging, at cost by quote (or user supplied) | By Quote | By Quote | By Quote | By Quote |
| Self operation and Staff time (per hour) | ||||
| Self operation tissue preparation, Cryostat and TM Sprayer (min 1/2 hr increments) | Hour | $10.00 | $60.00 | $100.00 |
| Self operation Amazon (min 1/2 hr increments) | Hour | $6.00 | $15.00 | $70.00 |
| Self operation MALDI TOF-TOF, Quantiva (min 1/2 hour increments) | Hour | $20.00 | $35.00 | $90.00 |
| Self operation QE-M, QE-P, Fusion, FT-ICR, Agilent, Exploris_480 (min 1/2 hour increments) | Hour | $25.00 | $50.00 | $120.00 |
| Self operation timsTOF Pro, timsTOF Flex (min 1/2 hour increments) | Hour | $25.00 | $50.00 | $120.00 |
| Staff time (consultation, sample/tissue prep, training, min 1/2 hour increments) | Hour | $55.00 | $110.00 | $220.00 |
| Proteomics | ||||
| 1D SDS-PAGE - 10 Wells | Sample | $65.00 | $110.00 | $200.00 |
| Albumin removal | Sample | $55.00 | $80.00 | $100.00 |
| Peptide/Protein Enrichment, e.g., Phosphorylation | Sample | $55.00 | $80.00 | $120.00 |
| Cell Lysis | Sample | $45.00 | $80.00 | $120.00 |
| Enzyme Digestion (in solution and in gel) | Digestion | $35.00 | $55.00 | $80.00 |
| S-trap digestion | Digestion | $50.00 | $60.00 | $80.00 |
| Protein Precipitation | Sample | $20.00 | $40.00 | $60.00 |
| Protein Quantitation (nanodrop), Peptide desalting | Sample | $15.00 | $35.00 | $80.00 |
| Off-line spin column fractionation or buffer exchange | Sample | $30.00 | $40.00 | $100.00 |
| Cryomill | Sample | $5.00 | $10.00 | $20.00 |
| Lyophylizer | Sample | $5.00 | $10.00 | $20.00 |
| Sample Analysis | ||||
| LC-MS/MS peptide sequencing (min 2 hour gradient) QE_P, Fusion, timsTOF Pro, Exploris_480 | Sample | $200.00 | $250.00 | $350.00 |
| LC-MS/MS peptide sequencing (max 1 hour gradient) QE_P, Fusion, timsTOF Pro, Exploris_480 | Sample | $150.00 | $180.00 | $250.00 |
| LC-MS/MS - 2D or off line LC Fractions per fraction | Fraction | $50.00 | $70.00 | $200.00 |
| Top-Down Proteomics (Fusion, QE-P, FT-ICR) | Sample | $300.00 | $500.00 | $900.00 |
| Quantitation (TMT labeling) – By Quote | By Quote | By Quote | By Quote | By Quote |
| Top Down Data Analysis (Fusion, QE_P, FT-ICR) | Sample | $300.00 | $500.00 | $900.00 |
| Native Mass Spectrometry | Sample | $55.00 | $60.00 | $100.00 |
| Custom Database Setup, basic search (per staff hour) | Hour | $55.00 | $110.00 | $220.00 |
| Additional Database Searches (per staff hour) | Hour | $55.00 | $110.00 | $220.00 |
| Consulting and Expensive Data Mining (per staff hour) | Hour | $55.00 | $110.00 | $220.00 |
Sample Submission Guidelines
Samples can be brought to 250 Biomedical Research Tower, along with a copy of your service request form, weekdays between 8 am and 5 pm. We have a refrigerator, freezer and dry box for samples that require special storage. It is preferred if samples are submitted neat or dried down, typically ug amounts is all that is needed.
Biological samples are handled differently and require different conditions. Salts, buffers and surfactants suppress ionization and need to be removed for mass spec analysis. When possible, it is advised to dialyze the sample into nanopure or at least distilled water prior to submission. We also have several de-salting techniques for proteins and oligonucleotides available in our facility. List all salts, buffers and detergents the protein or oligonucleotide is in, or has recently been in. For optimal results, please discuss your project with Dr. Somogyi prior to submission. Certain classes of proteins need to be handled differently than others, the more we know about your system, the better the data. Protein samples for ESI are prepared in 50% acetonitrile 50% water with 0.1% formic acid. Slightly acidic conditions are necessary to facilitate protonation
We CANNOT run samples that are in aromatic solvents like benzene, toluene, or solvents such as DMSO and DMF.
Electrospray and MALDI samples are prepared in Methanol, Acetonitrile, water, THF, chloroform. We will acidify the sample with acetic or formic acid unless you indicate the sample is acid sensitive.
Turn around times vary between 1 day to a week depending on sample load, instrument availability and type of analysis. We will email you when the results are ready.
Important notes about sample submissions
We utilize Facility Online Management (FOM) to manage our services. You will need a FOM account to submit your service request.
MSP user guide
A user guide with display logic is available at https://go.osu.edu/startusingmsp to guide MSP/PSR users through creating a FOM account and the sample submission process.
For External Users
Please submit information using the following link to request an OSU sponsored guest account for access to FOM. Request an OSU Sponsored Guest Account / FOM Profile and Account
Creating requisitions for use in FOM (internal users)
Instructions for creating a requisition for use in FOM and linking it to the FOM account are available at https://go.osu.edu/fomrequisition.
Electronic Service request Forms
When submitting multiple samples, please fill out only one (1) Service Request Form in FOM and attach all sample information in a separate document. Please do not submit multiple forms.
Please only submit service requests when you are ready to submit/send samples.
Need help?
If you run into problems with FOM please first troubleshoot using the MSP User Guide. If you require assistance, please contact fom-admin@osu.edu .